AssemblyTron.MoClo_builder package¶
Submodules¶
AssemblyTron.MoClo_builder.MoClo_writer module¶
Writer script for generating Modular cloning toolkit constructs assembly protocols in OpenTrons API.
This script performs Golden Gate assembly to build MoClo plasmids. Each part must come from a predetermined position in a 96 well plate. Each part is maintained as a plasmid, which should be purified prior to running this writer script.
This script runs on the command line of a personal laptop. It calls CSVs to make a customized protocol script that can be transferred directly to the OT-2 via the run app.
AssemblyTron.MoClo_builder.Setup_MoClo module¶
Setup script for building MoClo plasmids.
This script walks the user through selecting how many plasmids to build, which parts to include in the plasmids, and additional parameters.
This script requires no arguments, but instead obtains all necessary information and files by user-friendly tkinter pop-up windows.
This script requires pandas , numpy , shutil , tkinter to be installed in the python environment where running.
This script can also be called as a module by calling AssemblyTron.MoClo_builder.Setup_MoClo.